WWE canceled the scheduled three-hour-long live Raw show on June 25 (which, coincidentally, was supposed to be a memorial for the Mr. McMahon character), and replaced the broadcast version with a tribute to his life and career, featuring past matches, segments from the Hard Knocks: The Chris Benoit Story DVD , and comments from wrestlers and announcers from the Raw , SmackDown! , and ECW brands. Shortly after the program aired, many of the aired comments were posted on . It was not until the program was nearly over that reports surfaced that police were working under the belief that Benoit murdered his wife and son before killing himself.
Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  :10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity.