Gueck T, Aschenbach JR, Fuhrmann H.
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany. [email protected]
We investigated the influence of vitamin E on mediator activity and release in a canine mastocytoma cell line (C2) as a model for canine atopic dermatitis. Cells were incubated without and with vitamin E (100 microm) for 24 h. The histamine and prostaglandin D2 (PGD2) release as well as the chymase and tryptase activity were measured. To stimulate the PGD2 and histamine release, cells were incubated with the wasp venom peptide mastoparan (50 microm) for 30 or 45 min. Nonstimulated as well as mastoparan-stimulated histamine and PGD2 release was reduced significantly in vitamin E-treated cells. The activity of chymase tended to decrease, but the tryptase activity of C2 cells was not influenced by vitamin E. These results indicate that vitamin E decreased the production and release of inflammatory mediators in C2 cells, suggesting that vitamin E might have a possible beneficial effect in inflammatory diseases.
My Bernese is 9 years old and has a very large the (size of a grapefruit ) mast cell mass on his abdomen. He also has one the size of a golf ball and another just starting. Vet said she doesn’t like the looks of this. Started him on steroids and benadryl. He doesn’t act like himself but doesn’t seem to be hurting. He also has Lyme disease since he was a puppy. I do not think he can stand surgery, and if it even reasonable to consider this with multiple sites do I know if its time to let him go. I don’t want to be selfish and yet I cannot bear the pain of putting him down. I know I feel so blessed to have had my boy for this many years..a lot of Berner owners don’t get this time. Please, if you can help me put this into perspective.